Goat anti-NOTCH3 Antibody

Item#:

dAP-0751

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-NOTCH3 Antibody
Principle Name	NOTCH3
Official Symbol	NOTCH3
All Name and Symbols	NOTCH3; Notch homolog 3 (Drosophila); HGNC
Accession Number(s)	NP_000426.2
Peptide Sequence	QLGPQPEVTPKRQ, is from C Terminus
Human Gene ID(s)	4854
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa
Immunohistochemistry	In paraffin embedded Human Hippocampus shows select nuclear staining and weaker staining in different comparments of the neuronal cell bodies. Recommended concentration, 4-6µg/ml.
Reference(s)	Dang L, Fan X, Chaudhry A, Wang M, Gaiano N, Eberhart CG. Notch3 signaling initiates choroid plexus tumor formation. Oncogene. 2005 Sep 26; [Epub ahead of print] .PMID