Goat anti-Duffy / FY / DARC Antibody

Item#:

dAP-0744

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Duffy / FY / DARC Antibody
Principle Name	Duffy / FY / DARC
Official Symbol	ACKR1
All Name and Symbols	FY; DARC; HGNC
Accession Number(s)	NP_001116423.1; NP_002027.2
Peptide Sequence	HRAELSPSTENSSQLDFED, is from N Terminus
Human Gene ID(s)	2532
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC, FC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 37kDa band observed in Human Liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration
Reference(s)	Bruhl H, Vielhauer V, Weiss M, Mack M, Schlondorff D, Segerer S. Expression of DARC, CXCR3 and CCR5 in giant cell arteritis. Rheumatology (Oxford). 2005 Mar;44(3)