Goat anti-GGA3 Antibody |
| Principle Name | GGA3 |
| Official Symbol | GGA3 |
| All Name and Symbols | GGA3; golgi associated, gamma adaptin ear containing, ARF binding protein 3; HGNC |
| Accession Number(s) | NP_619525.1; NP_054720.1 |
| Peptide Sequence | EQLSTEVGEVDQ, is from C Terminus |
| Human Gene ID(s) | 23163 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_619525 and NP_054720) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave an approx 65kDa band in Mouse and Rat Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size |
| Immunohistochemistry | Paraffin embedded Human Kidney. Recommended concentration |
| Reference(s) | Prag G, Lee S, Mattera R, Arighi CN, Beach BM, Bonifacino JS, Hurley JH. Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding proteins. Proc Natl Acad Sci U S A. 2005 Feb 15;102(7) |
