Goat anti-VPS41 Antibody |
| Principle Name | VPS41 |
| Official Symbol | VPS41 |
| All Name and Symbols | VPS41; vacuolar protein sorting 41 (yeast) ; HGNC |
| Accession Number(s) | NP_055211.2; NP_542198.2 |
| Peptide Sequence | DHLKKDSQNKTLLK, is from internal region |
| Human Gene ID(s) | 27072 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_055211.2 and NP_542198.2) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe |
| Immunohistochemistry | In paraffin embedded Human Kidney shows staining of collective tubules. Recommended concentration, 4-6µg/ml. |
| Reference(s) | McVey Ward D, Radisky D, Scullion MA, Tuttle MS, Vaughn M, Kaplan J. hVPS41 is expressed in multiple isoforms and can associate with vesicles through a RING-H2 finger motif. Exp Cell Res. 2001 Jul 1;267(1) |
