Goat anti-SMURF1 Antibody

Item#:

dAP-0734

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-SMURF1 Antibody
Principle Name	SMURF1
Official Symbol	SMURF1
All Name and Symbols	SMURF1; SMAD specific E3 ubiquitin protein ligase 1; HGNC
Accession Number(s)	NP_065162.1; NP_851994.1
Peptide Sequence	PSEGSLEDEELPAQ, is from internal region
Human Gene ID(s)	57154
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms (NP_065162.1 and NP_851994.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 1000.
Western Blot	Preliminary experiments in lysates of cell lines HEK293, A431 and HepG2 gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been r
Reference(s)	Ozdamar B, Bose R, Barrios-Rodiles M, Wang HR, Zhang Y, Wrana JL. Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity. Science. 2005 Mar 11;307(5715)