Goat anti-VPS11 Antibody |
| Principle Name | VPS11 |
| Official Symbol | VPS11 |
| All Name and Symbols | VPS11; vacuolar protein sorting 11 (yeast) ; HGNC |
| Accession Number(s) | NP_068375.3 |
| Peptide Sequence | HALQEKDTQTKLE, is from internal region |
| Human Gene ID(s) | 55823 |
| Non-Human GeneID(s) | 71732 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the c |
| Immunohistochemistry | In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6µg/ml. |
| Reference(s) | Kim BY, Kramer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C. Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7. J Biol Chem. 2001 Aug 3;276(31) |
