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Goat anti-Klotho Antibody
Item#:
dAP-0726
Size Price Quantity
50.0 ug $310.00
Goat anti-Klotho Antibody
Principle Name Klotho
Official Symbol KL
All Name and Symbols KL; klotho
Accession Number(s) NP_004786.2; NP_710150.1
Peptide Sequence KRDDAKYMYYLKK, is from internal region
Human Gene ID(s) 9365
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported isoforms (NP_004786.2; NP_710150.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Preliminary experiments in Human Brain, Duodenum, Placenta and Prostate lysates gave no specific signal but low background at antibody concentration up to 1µg/ml.
Immunohistochemistry In paraffin embedded Human Breast shows cytoplasm staining in luminal epithelial cells of ducts, while Human Placenta shows membranous staining. Recommended concentration
Reference(s) Kurosu H, Yamamoto M, Clark JD, Pastor JV, Nandi A, Gurnani P, McGuinness OP, Chikuda H, Yamaguchi M, Kawaguchi H, Shimomura I, Takayama Y, Herz J, Kahn CR, Rosenblatt KP, Kuro-o M. Suppression of ageing in mice by the hormone Klotho. Science. 2005 Sep 16;309(5742)

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