Goat anti-SNM1 / DCLRE1A (internal) Antibody

Item#:

dAP-0724

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-SNM1 / DCLRE1A (internal) Antibody
Principle Name	SNM1 / DCLRE1A (internal)
Official Symbol	DCLRE1A
All Name and Symbols	DCLRE1A; DNA cross-link repair 1A (PSO2 homolog, S. cerevisiae); HGNC
Accession Number(s)	NP_055696.3
Peptide Sequence	PSPNQKRSSQCKRK, is from internal region
Human Gene ID(s)	9937
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Preliminary experiments gave an approx 26kDa band in human duodenum lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 117k
Immunohistochemistry	Paraffin embedded Human Skeletal Muscle. Recommended concentration
Reference(s)	Akhter S, Richie CT, Deng JM, Brey E, Zhang X, Patrick C Jr, Behringer RR, Legerski RJ. Deficiency in SNM1 abolishes an early mitotic checkpoint induced by spindle stress. Mol Cell Biol. 2004 Dec;24(23)