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Goat anti-CBX3 / HP1 Gamma Antibody
Item#:
dAP-0722
Size Price Quantity
50.0 ug $310.00
Goat anti-CBX3 / HP1 Gamma Antibody
Principle Name CBX3 / HP1 Gamma
Official Symbol CBX3
All Name and Symbols CBX3; HP1-GAMMA; chromobox homolog 3 (HP1 gamma homolog, Drosophila) ; HGNC
Accession Number(s) NP_009207.2
Peptide Sequence EAFLNSQKAGKEKD, is from internal region
Human Gene ID(s) 11335
Non-Human GeneID(s) 12417 (mouse); 297093 (rat);
Important Comments No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx. 20kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration
Immunohistochemistry In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3µg/ml.
Reference(s) Vakoc CR, Mandat SA, Olenchock BA, Blobel GA. Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin. Mol Cell. 2005 Aug 5;19(3)

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