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Goat anti-NHERF2 (isoform a) Antibody
Item#:
dAP-0719
Size Price Quantity
50.0 ug $310.00
Goat anti-NHERF2 (isoform a) Antibody
Principle Name NHERF2 (isoform a)
Official Symbol SLC9A3R2
All Name and Symbols SLC9A3R2; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2; HGNC
Accession Number(s) NP_001123484.1
Peptide Sequence DGSAWKQDPFQ, is from internal region
Human Gene ID(s) 9351
Non-Human GeneID(s)
Important Comments Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 38kDa band observed in human kidney lysates (calculated MW of 37.4kDa according to BAA33216.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Kidney shows strong membranous staining in glomerulus-. Recommended concentration, 1-2µg/ml.
Reference(s) Cha B, Kim JH, Hut H, Hogema BM, Nadarja J, Zizak M, Cavet M, Lee-Kwon W, Lohmann SM, Smolenski A, Tse CM, Yun C, de Jonge HR, Donowitz M. cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein. J Biol Chem. 2005 Apr 29;280(17)

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