Goat anti-VPS45 (Internal) Antibody

Item#:

dAP-0718

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-VPS45 (Internal) Antibody
Principle Name	VPS45 (Internal)
Official Symbol	VPS45
All Name and Symbols	VPS45A; HGNC
Accession Number(s)	NP_009190.2
Peptide Sequence	FQKKKPKEQQKLES, is from internal region
Human Gene ID(s)	11311
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 55kDa band observed in Human Placenta lysates (calculated MW of 65.1kDa according to NP_009190.2). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Spleen. Recommended concentration
Reference(s)	Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M. Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain. J Cell Biol. 2000 Oct 30;151(3)