Goat anti-Histamine Receptor H2 Antibody

Item#:

dAP-0715

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Histamine Receptor H2 Antibody
Principle Name	Histamine Receptor H2
Official Symbol	HRH2
All Name and Symbols	HRH2; histamine receptor H2; HGNC
Accession Number(s)	NP_071640.1; NP_001124527.1
Peptide Sequence	QEEKPLKLQVWSGTE, is from C Terminus
Human Gene ID(s)	3274
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC, IF
Tested Species	Rat
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Preliminary experiments gave bands at approx 55kDa and a stronger 28kDa in Human Colon and Duodenum lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observ
Immunohistochemistry	Immunofluorescence
Reference(s)	Nakane H, Sonobe Y, Watanabe T, Nakano K. Histamine