Goat anti-SIRT3 Antibody |
| Principle Name | SIRT3 |
| Official Symbol | SIRT3 |
| All Name and Symbols | SIRT3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae); HGNC |
| Accession Number(s) | NP_036371.1, NP_001017524.1 |
| Peptide Sequence | QRETGKLDGPDK, is from C Terminus |
| Human Gene ID(s) | 23410 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 28kDa band observed in Human Liver lysates (calculated MW of 28.6kDa according to NP_001017524.1). Recommended concentration |
| Reference(s) | Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes. J Biol Chem. 2005 Jan 14; [Epub ahead of print] .PMID |
