Goat anti-HMGA1 Antibody |
| Principle Name | HMGA1 |
| Official Symbol | HMGA1 |
| All Name and Symbols | HMGA1; high mobility group AT-hook 1 ; HGNC |
| Accession Number(s) | NP_665906.1; NP_665908.1; NP_002122.1; NP_665909.1; NP_665912.1; NP_665910.1 |
| Peptide Sequence | LASKQEKDGTEK, is from internal region |
| Human Gene ID(s) | 3159 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise isoform a (also called HMG-I; NP_665906.1; NP_665908.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave an approx 50kDa band in Human Testis and Ovary lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated si |
| Immunohistochemistry | In paraffin embedded Human Placenta shows strong nuclear staining. Recommended concentration, 4-6µg/ml. |
| Reference(s) | Chiappetta G, Botti G, Monaco M, Pasquinelli R, Pentimalli F, Di Bonito M,
D'Aiuto G, Fedele M, Iuliano R, Palmieri EA, Pierantoni GM, Giancotti V, Fusco
A. HMGA1 protein overexpression in human breast carcinomas |
