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Goat anti-Rhophilin 2 (Internal) Antibody
Item#:
dAP-0706
Size Price Quantity
50.0 ug $310.00
Goat anti-Rhophilin 2 (Internal) Antibody
Principle Name Rhophilin 2 (Internal)
Official Symbol RHPN2
All Name and Symbols RHPN2; p76RBE; rhophilin, Rho GTPase binding protein 2; rhophilin 2; rhophilin-2; RhoB effector; rhophilin-like protein; P76RBE; RHOBP; 76 kDa RhoB effector protein; GTP-Rho-binding protein 2; rhophilin-like Rho-GTPase binding protein
Accession Number(s) NP_149094.3
Peptide Sequence TNKNRQKSAST, is from internal region
Human Gene ID(s) 85415
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 35kDa band in Human Pancreas and Prostate lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculat
Immunohistochemistry In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10µg/ml.
Reference(s) Peck JW, Oberst M, Bouker KB, Bowden E, Burbelo PD. The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization. J Biol Chem. 2002 Nov 15;277(46)

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