Goat anti-Clusterin / APOJ Antibody

Item#:

dAP-0703

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Clusterin / APOJ Antibody
Principle Name	Clusterin / APOJ
Official Symbol	CLU
All Name and Symbols	CLU; APOJ; clusterin; complement lysis inhibitor; SP-40,40; sulfated glycoprotein 2; testosterone-repressed prostate message 2; apolipoprotein J ; HGNC
Accession Number(s)	NP_001822.2; NP_976084.1
Peptide Sequence	EKALQEYRKKHREE, is from C Terminus
Human Gene ID(s)	1191
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 35-37kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature (PMID
Reference(s)	Xie D, Lau SH, Sham JS, Wu QL, Fang Y, Liang LZ, Che LH, Zeng YX, Guan XY. Up-regulated expression of cytoplasmic clusterin in human ovarian carcinoma. Cancer. 2005 Jan 15;103(2)