Goat anti-NHERF2 (isoform b, e) Antibody

Item#:

dAP-0697

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-NHERF2 (isoform b, e) Antibody
Principle Name	NHERF2 (isoform b, e)
Official Symbol	SLC9A3R2
All Name and Symbols	SLC9A3R2; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2; HGNC
Accession Number(s)	NP_004776.3; NP_001239005.1
Peptide Sequence	DLPGSDKDTEESGLH, is from internal region
Human Gene ID(s)	9351
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize isoforms b and e (NP_004776.3 and NP_001239005.1) only.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 35kDa band observed in lysates of cell lines HEK293 and Daudi (calculated MW of 36.2kDa according to NP_004776.3). Recommended concentration
Reference(s)	Cha B, Kim JH, Hut H, Hogema BM, Nadarja J, Zizak M, Cavet M, Lee-Kwon W, Lohmann SM, Smolenski A, Tse CM, Yun C, de Jonge HR, Donowitz M. cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein. J Biol Chem. 2005 Apr 29;280(17)