Goat anti-GDF15 Antibody |
| Principle Name | GDF15 |
| Official Symbol | GDF15 |
| All Name and Symbols | GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; growth differentiation factor 15; PTGF-beta; prostate differentiation factor; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1 |
| Accession Number(s) | NP_004855.2 |
| Peptide Sequence | QKTDTGVSLQTYDD, is from C Terminus |
| Human Gene ID(s) | 9518 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Approx 35 kDa band observed in Human Prostate lysates (calculated MW of 34.1kDa according to NP_004855.2). Primary incubation was 1 hour. In transfected HEK293 transiently expressing GDF15 a band of approx 35 kDa is observed. This band is n |
| Immunohistochemistry | Paraffin embedded Human Prostate and Placenta. Recommended concentration |
| Reference(s) | Wollmann W, Goodman ML, Bhat-Nakshatri P, Kishimoto H, Goulet RJ Jr, Meharotra S, Morimiya A, Badve S, Nakshatri H. The macrophage inhibitory cytokine integrates AKT/PKB and MAP kinase signaling pathways in breast cancer cells. Carcinogenesis. 2005 Jan 27; [Epub ahead of print] .PMID |
