Goat anti-Osteopontin / SPP1 Antibody

Item#:

dAP-0689

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Osteopontin / SPP1 Antibody
Principle Name	Osteopontin / SPP1
Official Symbol	SPP1
All Name and Symbols	SPP1; HGNC
Accession Number(s)	NP_001035147.1; NP_000573.1; NP_001035149.1
Peptide Sequence	DPKSKEEDKHLK, is from C Terminus
Human Gene ID(s)	6696
Non-Human GeneID(s)	20750 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 60kDa band observed in lysates of Human Tonsil and lysates of cell line MOLT4 and Jurkat (calculated MW of 33.8kDa according to NP_000573.1). The observed molecular weight corresponds to earlier findings in literature (Singh et al,
Reference(s)	Kolb A, Kleeff J, Guweidhi A, Esposito I, Giese NA, Adwan H, Giese T, Buchler MW, Berger MR, Friess H. Osteopontin Influences the Invasiveness of Pancreatic Cancer Cells and is Increased in Neoplastic and Inflammatory Conditions. Cancer Biol Ther. 2005 Jul 5;4(7) [Epub ahead of print] .PMID