Goat anti-RAE1 Antibody |
| Principle Name | RAE1 |
| Official Symbol | RAE1 |
| All Name and Symbols | RAE1; RAE1 RNA export 1 homolog (S. pombe) ; HGNC |
| Accession Number(s) | NP_003601.1; NP_001015885.1 |
| Peptide Sequence | NAAEELKPRNKK, is from C Terminus |
| Human Gene ID(s) | 8480 |
| Non-Human GeneID(s) | |
| Important Comments | NP_003601and NP_001015885 represent identical proteins. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 40kDa band observed in lysates cell lines HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41.0kDa according to NP_003601.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Brain Cortex shows staining of neuronal cell bodies. Recommended concentration |
| Reference(s) | Blower MD, Nachury M, Heald R, Weis K. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell. 2005 Apr 22;121(2) |
