Goat anti-HNF4A Antibody |
| Principle Name | HNF4A |
| Official Symbol | HNF4A |
| All Name and Symbols | HNF4A; TCF; HNF4; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; hepatocyte nuclear factor 4, alpha; HNF4-alpha; transcription factor-14; FLJ39654; HNF4alpha; MODY; MODY1; HNF4alpha10/11/12; hepatic nuclear factor 4 alpha |
| Accession Number(s) | NP_849180.1; NP_000448.3; NP_849181.1 |
| Peptide Sequence | RLSKTLVDMDMADY, is from N Terminus |
| Human Gene ID(s) | 3172 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 55kDa and 48kDa bands observed in Mouse Small Intestine and in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1, 52.7kDa according to Mouse NP_032287.2 and |
| Immunohistochemistry | Paraffin embedded Human Small Intestine. Recommended concentration |
| Reference(s) | Damcott CM, Hoppman N, Ott SH, Reinhart LJ, Wang J, Pollin TI, O'Connell JR, Mitchell BD, Shuldiner AR. Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish. Diabetes. 2004 Dec;53(12) |
