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Goat anti-Spleen tyrosine kinase / SYK Antibody
Item#:
dAP-0673
Size Price Quantity
50.0 ug $310.00
Goat anti-Spleen tyrosine kinase / SYK Antibody
Principle Name Spleen tyrosine kinase / SYK
Official Symbol SYK
All Name and Symbols SYK; spleen tyrosine kinase; HGNC
Accession Number(s) NP_003168.2; NP_001128524.1
Peptide Sequence KYLQQNRHVKDKN, is from internal region
Human Gene ID(s) 6850
Non-Human GeneID(s) 20963 (mouse); 25155 (rat);
Important Comments This antibody is expected to recognise both reported isoforms (NP_003168.2; NP_001128524.1). Reported pairs of variants represent identical proteins (NP_003168.2 and NP_001167638.1; NP_001128524.1 and NP_001167639.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 70kDa band observed in Human Bone marrow, Lymph Node, and Spleen lysates (calculated MW of 72.1kDa according to NP_003168.2). Recommended concentration
Reference(s) Rogers NC, Slack EC, Edwards AD, Nolte MA, Schulz O, Schweighoffer E, Williams DL, Gordon S, Tybulewicz VL, Brown GD, Reis E Sousa C. Syk-dependent cytokine induction by Dectin-1 reveals a novel pattern recognition pathway for C type lectins. Immunity. 2005 Apr;22(4)

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