Goat anti-MIF Antibody |
| Principle Name | MIF |
| Official Symbol | MIF |
| All Name and Symbols | MIF; macrophage migration inhibitory factor (glycosylation-inhibiting factor) ; HGNC |
| Accession Number(s) | NP_002406.1 |
| Peptide Sequence | NAANVGWNNSTFA, is from C Terminus |
| Human Gene ID(s) | 4282 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC, EIA |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 13kDa band observed in Human Lymph Node, and Thymus lysates and similar bands are observed in lysates of cell lines Daudi and Jurkat (calculated MW of 12.5kDa according to NP_002406.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Thymus. Recommended concentration |
| Reference(s) | Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP. Obesity, macrophage migration inhibitory factor, and weight loss. Int J Obes Relat Metab Disord. 2005 Mar 29; [Epub ahead of print] .PMID |
