Goat anti-Makorin Antibody

Item#:

dAP-0664

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Makorin Antibody
Principle Name	Makorin
Official Symbol	MKRN1
All Name and Symbols	MKRN1; makorin; ring finger protein 1; HGNC
Accession Number(s)	NP_038474.1; NP_001138597.1
Peptide Sequence	VTKEESEKEQTAVET, is from internal region
Human Gene ID(s)	23608
Non-Human GeneID(s)
Important Comments	This antibody may cross react with MKRN4 (52.9kDa).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag a
Reference(s)	Kim JH, Park SM, Kang MR, Oh SY, Lee TH, Muller MT, Chung IK. Ubiquitin ligase MKRN1 modulates telomere length homeostasis through a proteolysis of hTERT. Genes Dev. 2005 Apr 1;19(7)