Goat anti-PUM2 Antibody |
| Principle Name | PUM2 |
| Official Symbol | PUM2 |
| All Name and Symbols | PUM2; PUMH2; PUML2; KIAA0235; pumilio homolog 2 (Drosophila); pumilio (Drosphila) homolog 2; FLJ36528; MGC138251; MGC138253; OTTHUMP00000200539; pumilio homolog 2 |
| Accession Number(s) | NP_056132.1 |
| Peptide Sequence | DQNRDLKQGDDDDSK, is from internal region |
| Human Gene ID(s) | 23369 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 4000. |
| Western Blot | In transfected HEK293 transiently expressing full-length Human PUM2 (myc and DYKDDDDK tagged), a band of approx. 140kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag a |
| Reference(s) | Szakmary A, Cox DN, Wang Z, Lin H. Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation. Curr Biol. 2005 Jan 26;15(2) |
