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Goat anti-RICTOR Antibody
Item#:
dAP-0646
Size Price Quantity
50.0 ug $310.00
Goat anti-RICTOR Antibody
Principle Name RICTOR
Official Symbol RICTOR
All Name and Symbols RICTOR; Rictor; KIAA1999; AVO3; MGC39830; likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae); rapamycin-insensitive companion of mTOR; mAVO3; pianissimo; DKFZp686B11164; RPTOR independent companion of MTOR, complex 2; PIA; mAVO3; TORC2-s
Accession Number(s) NP_689969.2
Peptide Sequence KQPIVDTSAES, is from C Terminus
Human Gene ID(s) 253260
Non-Human GeneID(s) 78757 (mouse);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in t
Immunohistochemistry In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3µg/ml.
Reference(s) Sarbassov dos D, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Curr Biol. 2004 Jul 27;14(14)

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