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Goat anti-AGRP / Agouti-related protein Antibody
Item#:
dAP-0644
Size Price Quantity
50.0 ug $310.00
Goat anti-AGRP / Agouti-related protein Antibody
Principle Name AGRP / Agouti-related protein
Official Symbol AGRP
All Name and Symbols AGRP; ART; AGRT; ASIP2; agouti related protein homolog (mouse); agouti (mouse) related protein; Agouti-related transcript, mouse, homolog of; MGC118963; agouti related protein homolog
Accession Number(s) NP_001129.1; NP_015531.1
Peptide Sequence CRKLGTAMNPCSRT, is from C Terminus
Human Gene ID(s) 181
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated s
Immunohistochemistry Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration
Reference(s) Breen TL, Conwell IM, Wardlaw SL. Effects of fasting, leptin, and insulin on AGRP and POMC peptide release in the hypothalamus. Brain Res. 2005 Jan 25;1032(1-2)

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