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Goat anti-Ymer / CCDC50 Antibody


Item#:
dAP-0643
Size Price Quantity
50.0 ug $310.00
Goat anti-Ymer / CCDC50 Antibody
Principle Name Ymer / CCDC50
Official Symbol CCDC50
All Name and Symbols CCDC50; Ymer protein; coiled-coil domain containing 50; C3orf6, YMER; C3orf6; chromosome 3 open reading frame 6; DFNA44
Accession Number(s) NP_848018.1; NP_777568.1
Peptide Sequence CFSKSESSHKGFHYK, is from internal region
Human Gene ID(s) 152137
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported isoforms of human Ymer protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 18kDa band in Human Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa
Immunohistochemistry In paraffin embedded Human Pancreas shows strong staining in cytoplasm in select acinar cells. Recommended concentration, 5-10µg/ml.
Reference(s) Blagoev B, Ong SE, Kratchmarova I, Mann M. Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat Biotechnol. 2004 Sep;22(9)

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