Goat anti-Lipocalin 2 / NGAL Antibody

Item#:

dAP-0638

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Lipocalin 2 / NGAL Antibody
Principle Name	Lipocalin 2 / NGAL
Official Symbol	LCN2
All Name and Symbols	LCN2; NGAL; lipocalin 2 (oncogene 24p3); HGNC
Accession Number(s)	NP_005555.2
Peptide Sequence	AILREDKDPQKMY, is from internal region
Human Gene ID(s)	3934
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 22kDa band observed in Human Kidney lysates (calculated MW of 22.6kDa according to NP_005555.2). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells. Recommended concentration
Reference(s)	Mishra J, Dent C, Tarabishi R, Mitsnefes MM, Ma Q, Kelly C, Ruff SM, Zahedi K, Shao M, Bean J, Mori K, Barasch J, Devarajan P. Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet. 2005 Apr;365(9466)