Goat anti-ERK1 / MAPK3 Antibody

Item#:

dAP-0636

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-ERK1 / MAPK3 Antibody
Principle Name	ERK1 / MAPK3
Official Symbol	MAPK3
All Name and Symbols	MAPK3; ERK1; mitogen-activated protein kinase 3; HGNC
Accession Number(s)	NP_002737.2; NP_001035145.1; NP_001103361.1
Peptide Sequence	GGEPRRTEGVGP, is from N Terminus
Human Gene ID(s)	5595
Non-Human GeneID(s)
Important Comments	No cross-reactivity expected with ERK2
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Approx 45kDa band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kDa according to NP_002737.1). Recommended concentration
Reference(s)	Wang PY, Weng J, Anderson RG. OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation. Science. 2005 Mar 4;307(5714)