Goat anti-BID Antibody |
| Principle Name | BID |
| Official Symbol | BID |
| All Name and Symbols | BID; BH3 interacting domain death agonist ; HGNC |
| Accession Number(s) | NP_932070.1; NP_001187.1; NP_932071.1 |
| Peptide Sequence | TYVRSLARNGMD, is from C Terminus |
| Human Gene ID(s) | 637 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1; NP_001187.1; NP_932071.1) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 26+24kDa bands observed in Human fibroblast A431 lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration |
| Reference(s) | Lund T, Stokke T, Olsen OE, Fodstad O. Garlic arrests MDA-MB-435 cancer cells in mitosis, phosphorylates the proapoptotic BH3-only protein Bim(EL) and induces apoptosis. Br J Cancer. 2005 Apr 12; [Epub ahead of print] .PMID |
