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Goat anti-GAD2 / GAD65 Antibody
Item#:
dAP-0630
Size Price Quantity
50.0 ug $310.00
Goat anti-GAD2 / GAD65 Antibody
Principle Name GAD2 / GAD65
Official Symbol GAD2
All Name and Symbols GAD2; GAD65; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 2 (pancreatic islets and brain, 65kD); RP11-420F12.2; MGC161605; MGC161607; Glutamate decarboxylase-2 (pancreas); gl
Accession Number(s) NP_000809.1; NP_001127838.1
Peptide Sequence TLEDNEERMSRLSK, is from internal region
Human Gene ID(s) 2572
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported variants.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 65kDa band observed in Human Brain (Cerebral Cortex, Cerebellum and Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration
Reference(s) Meyre D, Boutin P, Tounian A, Deweirder M, Aout M, Jouret B, Heude B, Weill J, Tauber M, Tounian P, Froguel P. Is Glutamate Decarboxylase 2 (GAD2) a genetic link between low birth weight and subsequent development of obesity in children? J Clin Endocrinol Metab. 2005 Jan 25; [Epub ahead of print] .PMID

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