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Goat anti-AKAP9 / AKAP450 / CG-NAP Antibody
Item#:
dAP-0617
Size Price Quantity
50.0 ug $310.00
Goat anti-AKAP9 / AKAP450 / CG-NAP Antibody
Principle Name AKAP9 / AKAP450 / CG-NAP
Official Symbol AKAP9
All Name and Symbols AKAP9; CG-NAP; PRKA9; YOTIAO; AKAP350; HYPERION; KIAA0803; A kinase (PRKA) anchor protein (yotiao) 9; AKAP120-like protein; kinase N-associated protein; A-kinase anchoring protein 450; A-kinase anchor protein, 350kDa; protein kinase A anchoring protein 9
Accession Number(s) NP_005742.4; NP_671714.1
Peptide Sequence SGSTTQFHAGMR, is from C Terminus
Human Gene ID(s) 10142
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IF
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot This product has been successfully used by a customer, showing a band at approx 450kDa in lysates of cell line HepG2 (calculated MW of 453kDa according to NP_005742.4). Recommended concentration
Reference(s) Ciampi R, Knauf JA, Kerler R, Gandhi M, Zhu Z, Nikiforova MN, Rabes HM, Fagin JA, Nikiforov YE. Oncogenic AKAP9-BRAF fusion is a novel mechanism of MAPK pathway activation in thyroid cancer. J Clin Invest. 2005 Jan;115(1)

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