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Goat anti-BOULE / BOLL Antibody
Item#:
dAP-0606
Size Price Quantity
50.0 ug $310.00
Goat anti-BOULE / BOLL Antibody
Principle Name BOULE / BOLL
Official Symbol BOLL
All Name and Symbols BOLL; bol, boule-like (Drosophila); BOULE; boule
Accession Number(s) NP_932074.1; NP_149019.1
Peptide Sequence QPEPIKTVWSIHY, is from C Terminus
Human Gene ID(s) 66037
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize both reported isoforms (NP_932074.1; NP_149019.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.
Immunohistochemistry In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6µg/ml.
Reference(s) Xu EY, Moore FL, Pera RA. A gene family required for human germ cell development evolved from an ancient meiotic gene conserved in metazoans. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13)

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