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Goat anti-CD274 / PD-L1 Antibody
Item#:
dAP-0602
Size Price Quantity
50.0 ug $310.00
Goat anti-CD274 / PD-L1 Antibody
Principle Name CD274 / PD-L1
Official Symbol CD274
All Name and Symbols CD274; CD274 antigen; PD-L1; PDCD1LG1; B7-H; B7H1; PDL1; PDCD1L1; programmed cell death 1 ligand 1; PDL1; HGNC
Accession Number(s) NP_054862.1; NP_001254635.1
Peptide Sequence CKKQSDTHLEET, is from C Terminus
Human Gene ID(s) 29126
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize reported isoforms a and b (NP_054862.1; NP_001254635.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 40kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). The observed molecular weight corresponds to the glycosylated forms. Recommended concentration
Immunohistochemistry In paraffin embedded Human Placenta shows membranous staining of cytotrophoblasts. Recommended concentration
Reference(s) Thompson RH, Gillett MD, Cheville JC, Lohse CM, Dong H, Webster WS, Krejci KG, Lobo JR, Sengupta S, Chen L, Zincke H, Blute ML, Strome SE, Leibovich BC, Kwon ED. Costimulatory B7-H1 in renal cell carcinoma patients

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