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Goat anti-SEMA3E Antibody


Item#:
dAP-0593
Size Price Quantity
50.0 ug $310.00
Goat anti-SEMA3E Antibody
Principle Name SEMA3E
Official Symbol SEMA3E
All Name and Symbols SEMA3E; SEMAH; coll-5; M-SEMAH; M-SemaK; KIAA0331; M-sema H; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E
Accession Number(s) NP_036563.1
Peptide Sequence KPEHYRLPRHTLDS, is from C Terminus
Human Gene ID(s) 9723
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Preliminary experiments gave bands at approx 35kDa and 20kDa in Human Prostate lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculate
Immunohistochemistry In paraffin embedded Human Adrenal Medulla shows granular cytoplasmic staining increasing towards the zona reticularis. Recommended concentration, 2-4µg/ml.
Reference(s) Gu C, Yoshida Y, Livet J, Reimert DV, Mann F, Merte J, Henderson CE, Jessell TM, Kolodkin AL, Ginty DD. Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins. Science. 2005 Jan 14;307(5707)

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