Goat anti-CASP4 Antibody |
| Principle Name | CASP4 |
| Official Symbol | CASP4 |
| All Name and Symbols | CASP4; TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II; caspase 4, apoptosis-related cysteine protease; apoptotic cysteine protease Mih1/TX; caspase 4 |
| Accession Number(s) | NP_001216.1; NP_150649.1 |
| Peptide Sequence | CKERAEEIYPIKERN, is from internal region |
| Human Gene ID(s) | 837 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP_150649.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 40kDa band observed in Human Heart lysates (calculated MW of 43.3kDa according to NP_001216.1). Recommended concentration |
| Reference(s) | Munday NA, Vaillancourt JP, Ali A, Casano FJ, Miller DK, Molineaux SM, Yamin TT, Yu VL, Nicholson DW. Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases. J Biol Chem. 1995 Jun 30;270(26) |