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Goat anti-ERK2 / MAPK1 Antibody
Item#:
dAP-0586
Size Price Quantity
50.0 ug $310.00
Goat anti-ERK2 / MAPK1 Antibody
Principle Name ERK2 / MAPK1
Official Symbol MAPK1
All Name and Symbols MAPK1; ERK2; ERK; p38; p40; p41; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; mitogen-activated protein kinase 1; protein tyrosine kinase ERK2; mitogen-activated protein kinase 2; extracellular signal-regulated kinase 2; extracellular signal-regulated kin
Accession Number(s) NP_002736.3; NP_620407.1
Peptide Sequence CAAGPEMVRGQVF, is from internal region
Human Gene ID(s) 5594
Non-Human GeneID(s) 26413 (mouse);
Important Comments This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot Approx 38kDa band observed in human cervix epitheloid carcinoma HeLa and and human hepatoblastoma HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration
Reference(s) Lee SM, Nguyen TH, Park MH, Kim KS, Cho KJ, Moon DC, Kim HY, Yoon do Y, Hong JT. EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes. Biochem Biophys Res Commun. 2004 Aug 6;320(4)

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