Goat anti-SPHAR Antibody |
| Principle Name | SPHAR |
| Official Symbol | SPHAR |
| All Name and Symbols | SPHAR; S-phase response (cyclin-related); MGC88280; RP4-803J11.4; S-phase response (cyclin related) |
| Accession Number(s) | NP_006533.1 |
| Peptide Sequence | NVSFMIDLQAAHF, is from C Terminus |
| Human Gene ID(s) | 10638 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 7.5kDa according to NP_006533. Therefore we cannot recommend an optimal concentration and the produ |
| Reference(s) | Digweed M, Gunthert U, Schneider R, Seyschab H, Friedl R, Sperling K. Irreversible repression of DNA synthesis in Fanconi anemia cells is alleviated by the product of a novel cyclin-related gene. Mol Cell Biol. 1995 Jan;15(1) |
