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Goat anti-XAGE1 (Isoform 1d) / GAGED2 Antibody


Item#:
dAP-0579
Size Price Quantity
50.0 ug $310.00
Goat anti-XAGE1 (Isoform 1d) / GAGED2 Antibody
Principle Name XAGE1 (Isoform 1d) / GAGED2
Official Symbol GAGED2
All Name and Symbols GAGED2; XAGE1; XAGE-1; G antigen, family D, 2; XAGE-1 protein
Accession Number(s) NP_597673.1
Peptide Sequence CGFGFRRQGEDNT, is from C Terminus
Human Gene ID(s) 9503
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the produ
Immunohistochemistry In paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes. Recommended concentration, 3-6µg/ml.
Reference(s) Liu XF, Helman LJ, Yeung C, Bera TK, Lee B, Pastan I. XAGE-1, a new gene that is frequently expressed in Ewing's sarcoma. Cancer Res. 2000 Sep 1;60(17)

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