Goat anti-Centromere protein E Antibody

Item#:

dAP-0578

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Centromere protein E Antibody
Principle Name	Centromere protein E
Official Symbol	CENPE
All Name and Symbols	CENPE; CENP-E; centromere protein E (312kD); Centromere autoantigen E (312kD); centromere protein E, 312kDa; KIF10; centromere protein E; kinesin family member 10
Accession Number(s)	NP_001804.2
Peptide Sequence	HASSGKDVPECKTQ, is from C Terminus
Human Gene ID(s)	1062
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 316kDa according to NP_001804. Therefore we cannot recommend an optimal concentration and the produ
Reference(s)	Yen TJ, Compton DA, Wise D, Zinkowski RP, Brinkley BR, Earnshaw WC, Cleveland DW. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase. EMBO J. 1991 May;10(5)