Goat anti-Choline acetyltransferase Antibody

Item#:

dAP-0576

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Choline acetyltransferase Antibody
Principle Name	Choline acetyltransferase
Official Symbol	CHAT
All Name and Symbols	CHAT; choline acetyltransferase; CMS1A; CMS1A2; acetyl CoA
Accession Number(s)	NP_066264.3; NP_065574.3; NP_001136405.1
Peptide Sequence	CKEKATRPSQGHQP, is from C Terminus
Human Gene ID(s)	1103
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1). Reported variants represent identical protein (NP_066264.3; NP_066265.3; NP_066266.3; NP_001136406.1; NP_001136401.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 70kDa band observed in Human Placenta lysates (calculated MW of 70.5kDa according to NP_065574 and NP_066264). Recommended concentration
Reference(s)	Madziar B, Lopez-Coviella I, Zemelko V, Berse B. Regulation of cholinergic gene expression by nerve growth factor depends on the phosphatidylinositol-3'-kinase pathway. J Neurochem. 2005 Feb;92(4)