Goat anti-PAD4 / PADI4 Antibody

Item#:

dAP-0562

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PAD4 / PADI4 Antibody
Principle Name	PAD4 / PADI4
Official Symbol	PADI4
All Name and Symbols	PADI4; PAD; PDI4; PDI5; PADI5; peptidyl arginine deiminase, type IV; protein-arginine deiminase; peptidyl arginine deiminase, type V; PAD4; HL-60 PAD; PADI-H protein
Accession Number(s)	NP_036519.2
Peptide Sequence	AQGTLIRVTPEQPC, is from N Terminus
Human Gene ID(s)	23569
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 74kDa band observed in Human Spleen lysates (calculated MW of 74.1kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx. 75kDa is observed. This band is not observed in the non-transfected
Reference(s)	Wang Y, Wysocka J, Sayegh J, Lee YH, Perlin JR, Leonelli L, Sonbuchner LS, McDonald CH, Cook RG, Dou Y, Roeder RG, Clarke S, Stallcup MR, Allis CD, Coonrod SA. Human PAD4 regulates histone arginine methylation levels via demethylimination. Science. 2004 Oct 8;306(5694)