Goat anti-ARHGEF4 Antibody

Item#:

dAP-0561

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-ARHGEF4 Antibody
Principle Name	ARHGEF4
Official Symbol	ARHGEF4
All Name and Symbols	ARHGEF4; Rho guanine nucleotide exchange factor (GEF) 4; ASEF; GEF4; STM6; KIAA1112; DKFZp434G2016; APC-stimulated guanine nucleotide exchange factor; ASEF1; Rho guanine nucleotide exchange factor 4
Accession Number(s)	NP_056135.2; NP_127462.1
Peptide Sequence	PWEEPAGEKPSCS, is from N Terminus
Human Gene ID(s)	50649
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 80kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1kDa according to NP_056135.2 and 76.4kDa according to 127462.1). Recommended concentration
Reference(s)	Thiesen S, Kubart S, Ropers HH, Nothwang HG. Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in 3p13-21 and 2q22. Biochem Biophys Res Commun. 2000 Jun 24;273(1)