Goat anti-AKT3 Antibody |
| Principle Name | AKT3 |
| Official Symbol | AKT3 |
| All Name and Symbols | AKT3; PKBG; PRKBG; STK-2; RAC-gamma; RAC-PK-gamma; DKFZP434N0250; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); protein kinase B gamma; serine threonine protein kinase, Akt-3; RAC-gamma serine/threonine protein kinase; PKB-GAMMA |
| Accession Number(s) | NP_005456.1; NP_859029.1 |
| Peptide Sequence | CSPTSQIDNIGEEEM, is from internal region |
| Human Gene ID(s) | 10000 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1; NP_001193658.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | A 50-55kDa band observed in lysates of cell lines HepG2 and Jurkat and in Mouse and Rat Brain lysates (calculated MW of 54.0kDa according to NP_859029.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Prostate and Heart. Recommended concentration |
| Reference(s) | Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR. AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. Clin Cancer Res. 2001 Aug;7(8) |
