Goat anti-LRP6 Antibody |
| Principle Name | LRP6 |
| Official Symbol | LRP6 |
| All Name and Symbols | LRP6; low density lipoprotein receptor-related protein 6; low density lipoprotein-related protein 6; ADCAD2; FLJ90062; FLJ90421 |
| Accession Number(s) | NP_002327.2 |
| Peptide Sequence | CMTSVATAKGYTSDL, is from internal region |
| Human Gene ID(s) | 4040 |
| Non-Human GeneID(s) | 16974 (mouse); |
| Important Comments | This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Preliminary experiments gave bands at approx 50kDa and 35kDa in Human Adipose lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated |
| Immunohistochemistry | In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration |
| Reference(s) | Tamai K, Semenov M, Kato Y, Spokony R, Liu C, Katsuyama Y, Hess F, Saint-Jeannet JP, He X. LDL-receptor-related proteins in Wnt signal transduction. Nature. 2000 Sep 28;407(6803) |
