Goat anti-DOK5 Antibody |
| Principle Name | DOK5 |
| Official Symbol | DOK5 |
| All Name and Symbols | DOK5; MGC16926; C20orf180; docking protein 5; downstream of tyrosine kinase 5; chromosome 20 open reading frame 180 |
| Accession Number(s) | NP_060901.2 |
| Peptide Sequence | CLHRTETFPAYRSEH, is from C Terminus |
| Human Gene ID(s) | 55816 |
| Non-Human GeneID(s) | 76829 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | In transfected HEK293 transiently expressing DOK5 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 35.5kDa according to NP_060901.2. Recommended concentration, 1- |
| Immunohistochemistry | In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells. Recommended concentration, 1-2µg/ml. |
| Reference(s) | Cai D, Dhe-Paganon S, Melendez PA, Lee J, Shoelson SE. Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5. J Biol Chem. 2003 Jul 11;278(28) |
