Goat anti-PTEN Antibody |
| Principle Name | PTEN |
| Official Symbol | PTEN |
| All Name and Symbols | PTEN; BZS; MHAM; TEP1; MMAC1; PTEN1; MGC11227; phosphatase and tensin homolog (mutated in multiple advanced cancers 1); tensin homolog; mutated in multiple advanced cancers 1; MMAC1 phosphatase and tension homolog deleted on chromosome 10; phosphatase and |
| Accession Number(s) | NP_000305.3 |
| Peptide Sequence | CNEPFDEDQHTQIT, is from internal region (near the C Terminus) |
| Human Gene ID(s) | 5728 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IP |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 55kDa band observed in lysates of cell line A431 and 50kDa in lysates of cell line NIH3T3 (calculated MW of 47.1kDa according to Human NP_000305 and Mouse NP_032986.1). Recommended concentration |
| Reference(s) | Cantley LC, Neel BG. New insights into tumor suppression |
