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Goat anti-FHL1 / SLIM1 Antibody
Item#:
dAP-0542
Size Price Quantity
50.0 ug $310.00
Goat anti-FHL1 / SLIM1 Antibody
Principle Name FHL1 / SLIM1
Official Symbol FHL1
All Name and Symbols FHL1; SLIM1; KYO-T; RP11-535K18.1; FHL1A; four and a half LIM domains 1; Four-and-a-half LIM domains 1; skeletal muscle LIM-protein1; FHL1B; KYOT; MGC111107; XMPMA; SLIMMER; LIM protein SLIMMER; four-and-a-half Lin11; Isl-1 and Mec-3 domains 1; skeletal m
Accession Number(s) NP_001440.2; NP_001153171.1
Peptide Sequence CNKRFVFHNEQVY, is from internal region
Human Gene ID(s) 2273
Non-Human GeneID(s) 14199 (mouse); 25177 (rat);
Important Comments This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 30kDa band observed in Human Muscle lysates (calculated MW of 31.9kDa according to NP_001440). Recommended concentration
Immunohistochemistry In paraffin embedded Human Testis shows fibrous staining in the cytoplasm of developing sperm cells.. Recommended concentration
Reference(s) Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear J Biol Chem. 1999 Sep 17;274(38)

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