Goat anti-PTPRT Antibody |
| Principle Name | PTPRT |
| Official Symbol | PTPRT |
| All Name and Symbols | PTPRT; RPTPrho; KIAA0283; protein tyrosine phosphatase, receptor type, T; receptor protein tyrosine phosphatase; RP5-1121H13.2 |
| Accession Number(s) | NP_573400.2; NP_008981.3 |
| Peptide Sequence | CNTWEKPMLDQAV, is from internal region |
| Human Gene ID(s) | 11122 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise both reported isoforms. Epitope corresponding to aa 58-69 of human PTPRT protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 150kDa band observed in Human Brain lysates (calculated MW of 164kDa according to NP_573400.2 and 163kDa according to NP_008981.3). Recommended concentration |
| Reference(s) | Wang Z, Shen D, Parsons DW, Bardelli A, Sager J, Szabo S, Ptak J, Silliman N, Peters BA, van der Heijden MS, Parmigiani G, Yan H, Wang TL, Riggins G, Powell SM, Willson JK, Markowitz S, Kinzler KW, Vogelstein B, Velculescu VE. Mutational analysis of the tyrosine phosphatome in colorectal cancers. Science. 2004 May 21;304(5674) |
